Dilution-PCR method for wide-range quantitation of HBV DNA.
نویسندگان
چکیده
منابع مشابه
Is Quantitative HBsAg Measurement a Reliable Substitute for HBV DNA Quantitation?
Background: Hepatitis B surface antigen (HBsAg) is one of the main proteins of HBV envelop and its serum quantitative measurement is the most common quantitative test for monitoring the progress of Chronic Hepatitis B. Although measurement of serum HBV DNA copy number is a gold standard method for displaying viral load, the test is relatively expensive and it is not readily available everywhere...
متن کاملبررسی غلظت بتا دومیکروگلوبولین سرمی درافراد HBV DNA PCR+، HBsAg+و-HBV DNA PCR ، HBsAg+: به عنوان نشانگر تکثیر ویروس
چکید ه سابقه و هدف بتادومیکروگلوبولین ( b 2MG) ، زنجیره سبک آنتیژن MHC-I میباشد که غلظت سرمی آن ( S β 2MG ) در افراد سالم کمتر از mg/L 3 ذکر شده است. در عفونتهای هپاتیتی، عرضه آنتیژنهای ویروسی سطح هپاتوسیتها روی آنتیژنهای MHC-I در حذف ویروس نقش مهمی ایفا میکند. مواد و روشها در این مطالعه توصیفی درسرم 4 9 بیمار مبتلا به هپاتیت B در مقایسه با 3 5 فرد کنترل با سرولوژی منفی (فا...
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Previously, we designed an internally controlled quantitative nested real-time (QNRT) PCR assay for Mycobacterium tuberculosis DNA in order to rapidly diagnose tuberculous meningitis. This technique combined the high sensitivity of nested PCR with the accurate quantification of real-time PCR. In this study, we attempted to improve the original QNRT-PCR assay and newly developed the wide-range Q...
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AIM The aim of this study was to determine the concentration of salivary B2M as a marker of viral proliferation in HBS Ag(+), HBV DNA PCR(+) and Hbs Ag(+) and HBV DNA PCR(-) subjects. BACKGROUND Beta-2 microglobulin (B2M) is responsible for transmission of viral antigens such as Hepatitis B (HBV) on the surface of liver cells as part of an HLA complex. PATIENTS AND METHODS In this case cont...
متن کاملReal time TaqMan PCR detection and quantitation of HBV genotypes A-G with the use of an internal quantitation standard.
BACKGROUND Diagnostic assays for the accurate quantitation of hepatitis B virus (HBV) DNA levels from patients undergoing antiviral therapy are useful for monitoring and tailoring therapy. Such assays should give accurate results with all HBV genotypes, including a seventh genotype of hepatitis B virus, genotype G, that has recently been identified in specimens from HBV-positive patients in the...
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ژورنال
عنوان ژورنال: Kanzo
سال: 2002
ISSN: 0451-4203,1881-3593
DOI: 10.2957/kanzo.43.482